体液
生物
计算生物学
DNA
信使核糖核酸
鼻粘膜
背景(考古学)
法医鉴定
鉴定(生物学)
犯罪现场
DNA分析
核糖核酸
仿形(计算机编程)
DNA测序
基因
病理
遗传学
免疫学
医学
计算机科学
心理学
操作系统
古生物学
犯罪学
植物
作者
Olivia Chirnside,Anna Lemalu,Rachel Fleming
标识
DOI:10.1016/j.fsigen.2020.102317
摘要
Biological fluids are commonly encountered as a form of evidence within forensic science, and can often provide important information relating to events which may have occurred. Over the years, significant advancements have been made with DNA profiling techniques, allowing for links to be made between an individual and cellular material recovered from a crime scene. While this DNA analysis can aid in linking an individual to a crime, it can often be beneficial to also determine the body fluid source of the DNA obtained from the sample in question for case context. One increasing area within the forensic field is the use of mRNA profiling for the identification of body fluids. The analysis of gene expression patterns can give information on cell function, and ultimately the body fluid source of the DNA in a sample. Over time this has led to the development of mRNA reverse transcriptase PCR assays to detect body fluid specific RNA transcripts for casework. During the use of these techniques nasal mucosa has been observed to give rise to false positive results. We report here on the identification of promising markers using RNA sequencing for the detection of nasal mucosa, with the aim to incorporate these markers into existing assays to assist in the identification of nasal mucosa and to assist in the interpretation of possible false positive results.
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