QUANTITATIVE DETERMINATION OF PARACETAMOL, CAFFEINE AND CODEINE PHOSPHATE IN PHARMACEUTICAL DOSAGE FORMS BY USING CAPILLARY ELECTROPHORESIS METHOD

A new capillary electrophoresis method has been developed to analysis of paracetamol (PAR), caffeine (CAF) and codeine (COD) phosphate from pharmaceutical dosage forms.Good results were obtained by using a background electrolyte solution consist of 25 mM (pH 8.5) phosphate buffer solution containing 10% methanol.The separation was performed through an uncoated fused-silica capillaries (50 μm internal diameter, 56 cm total length, 47.5 cm effective length) at 25ºC with the application of 10 seconds of hydrodynamic injection at 40 mbar pressure and a potential of 27 kV.Detection wavelength was chosen 210 nm.Under optimum conditions, the migration times were found 4.427 min for PAR, 3.952 min for CAF, and 3.253 min for COD-phosphate.Linearity ranges were determined 99.72 -1994.40μg mL -1 for PAR, 5.75 -115.04 μg mL -1 for CAF and 1.84 -36.80 μg mL -1 for COD-phosphate for the method.Limit of detections were found 0.724 μg mL -1 for PAR, 0.533 μg mL -1 for CAF and 0.263 μg mL -1 for COD-phosphate in this study.Also, limit of quantitations were found 2.415 μg mL -1 for PAR, 1.776 μg mL -1 for CAF and 0.877 μg mL -1 for COD-phosphate.According to the obtained results, the method is sensitive, specific and repeatable to analysis of PAR, CAF and COD-phosphate.