刺激
成骨细胞
细胞生长
骨愈合
PI3K/AKT/mTOR通路
细胞生物学
生物电磁学
溴脱氧尿苷
碱性磷酸酶
化学
医学
内分泌学
信号转导
生物
电磁场
体外
解剖
生物化学
物理
酶
量子力学
作者
Hironori Miyamoto,Yasunobu Sawaji,Takahiro Iwaki,Toshinori Masaoka,Eiichi Fukada,Munehiro Date,Kengo Yamamoto
摘要
Pulsed electromagnetic fields (PEMFs) have been shown to be a noninvasive physical stimulant for bone fracture healing. However, PEMF stimulation requires a relatively long period of time and its mechanism of action has not yet been fully clarified. Recently, the mammalian target of rapamycin (mTOR) pathway has been shown to be involved in bone formation. This study aimed to investigate the effects of PEMFs on osteoblastic MC3T3-E1 cells by examining various cellular responses including changes in the mTOR pathway. Continuous PEMF stimulation induced a transient phosphorylation of the mTOR pathway, whereas intermittent PEMF stimulation (1 cycle of 10 min stimulation followed by 20 min of stimulation pause) revitalized the reduced phosphorylation. Moreover, PEMF stimulation stimulated cell proliferation (bromodeoxyuridine incorporation) rather than differentiation (alkaline phosphatase activity), with a more notable effect in the intermittently stimulated cells. These results suggest that intermittent PEMF stimulation may be effective in promoting bone fracture healing by accelerating cell proliferation, and in shortening stimulation time. Bioelectromagnetics. 2019;40:412-421. © 2019 Bioelectromagnetics Society.
科研通智能强力驱动
Strongly Powered by AbleSci AI