Highly sensitive and selective photoelectrochemical aptasensing of di-2-ethylhexyl phthalate based on graphene quantum dots decorated TiO2 nanotube arrays

A photoelectrochemical (PEC) sensing platform for di-2-ethylhexyl phthalate (DEHP) was constructed using graphene quantum dots decorated TiO2 nanotube arrays (GQDs-decorated TiO2 NTs) as the transducer species and the anti-DEHP aptamer as the biological recognition element. GQDs were synthesized using the alkali-mediated hydrothermal method, and then anchored onto the TiO2 NTs uniformly and intimately via pronounced electrostatic interaction. Coupling GQDs with TiO2 NTs not only enhanced visible-light absorption, but promoted charge separation and transportation, exhibiting excellent photocurrent response, and PEC activity. Various means were conducted to explore morphologies, optical, structural and PEC properties of the materials. As an identification unit, the anti-DEHP aptamer molecules were immobilized on GQDs-decorated TiO2 NTs using a cross-linking coupling method. The developed PEC sensing platform exhibits excellent sensing behavior for DEHP, and provides a low detection limit of 0.1 ng/L, high selectivity and stability. Meanwhile, its application in real environmental samples was evaluated and satisfying results were achieved. Thus, the established sensing platform provides a promising tool to detect DEHP in the environment.