Calcium phosphate-based biomaterials trigger human macrophages to release extracellular traps

生物材料 材料科学 细胞外 生物物理学 单核细胞 巨噬细胞 免疫系统 细胞生物学 体外 化学 免疫学 纳米技术 生物化学 生物 冶金
作者
Annika Seifert,Tina Tylek,Carina Blum,Naomi Hemmelmann,Bettina Böttcher,Uwe Gbureck,Jürgen Gröll
出处
期刊:Biomaterials [Elsevier]
卷期号:285: 121521-121521 被引量:15
标识
DOI:10.1016/j.biomaterials.2022.121521
摘要

As central part of the innate immune response, immune cells fight against invaders through various mechanisms, such as the release of extracellular traps (ETs). While this mechanism is mainly known for neutrophils in biomaterial contact, the release of macrophage extracellular traps (METs) in response to biomaterials has not yet been reported. An important application area for biomaterials is bone, where healing of defects of a critical size requires the implantation of grafts, which are often composed of calcium phosphates (CaPs). In this study, the response of human monocyte-derived macrophages in vitro to two different CaPs (α-tricalcium phosphate (α-TCP) and calcium deficient hydroxyapatite (CDHA)) as well as different pore structures was investigated. Scaffolds with anisotropic porosity were prepared by directional freezing, while samples with isotropic pore structure served as reference. It was revealed that ETs are released by human monocyte-derived macrophages in direct or indirect contact with CaP scaffolds. This was caused by mineral nanoparticles formed during incubation of α-TCP samples in culture medium supplemented with human platelet lysate, with an anisotropic pore structure attenuating MET formation. METs were significantly less pronounced or absent in association with CDHA samples. It was furthermore demonstrated that MET formation was accompanied by an increase in pro-inflammatory cytokines. Thus, this study provided the first evidence that macrophages are capable of releasing ETs in response to biomaterials.
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