去细胞化
再生(生物学)
坐骨神经
神经导管
脚手架
静电纺丝
生物医学工程
神经纤维
周围神经
神经外膜修复
组织工程
解剖
体内
髓鞘
基质(化学分析)
细胞外基质
化学
材料科学
医学
细胞生物学
中枢神经系统
复合材料
生物
聚合物
内科学
生物技术
生物化学
作者
Yan Kong,Jiawei Xu,Qi Han,Tiantian Zheng,Linliang Wu,Guicai Li,Yumin Yang
标识
DOI:10.1016/j.ijbiomac.2022.04.161
摘要
The composition and spatial structure of bioscaffold materials are essential for constructing tissue regeneration microenvironments. In this study, by using an electrospinning technique without any other additives, we successfully developed pure porcine decellularized nerve matrix (xDNME) conduits. The developed xDNME was composed of an obvious decellularized matrix fiber structure and effectively retained the natural components in the decellularized matrix of the nerve tissue. The xDNME conduit exhibited superior biocompatibility and the ability to overcome inter-species barriers. In vivo, after 12 weeks of implantation, xDNME significantly promoted the regeneration of rat sciatic nerve. The regenerated nerve fibers completely connected the two ends of the nerve defect, which were about 8 mm apart. The xDNME and xDNME-OPC groups showed myelin structures in the regenerated nerve fibers. In the xDNME group, the average thickness of the regenerated myelin sheath was 0.640 ± 0.013 μm, which was almost comparable to that in the autologous nerve group (0.646 ± 0.017 μm). Electrophysiological experiments revealed that both of the regenerated nerve fibers in the xDNME and xDNME-OPC groups had excellent abilities to transmit electrical signals. Respectively, the average conduction velocities of xDNME and xDNME-OPC were 8.86 ± 3.57 m/s and 6.99 ± 3.43 m/s. In conclusion, the xDNME conduits have a great potential for clinical treatment of peripheral nerve injuries, which may clinically transform peripheral nerve related regenerative medicine.
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