甜菜碱
脂肪变性
脂质代谢
基因
生物
翻译(生物学)
脂肪肝
基因表达
生物化学
转录组
信使核糖核酸
内分泌学
内科学
医学
疾病
作者
Tengda Huang,Jingsu Yu,Zupeng Luo,Lin Yu,Siqi Liu,Peng Wang,Mengting Jia,Tian Wu,Weiwei Miao,Lei Zhou,Ziyi Song,Haojie Zhang,Youguo Li
标识
DOI:10.1016/j.bbrc.2021.08.058
摘要
Non-alcoholic fatty liver disease (NAFLD) is a common disease with a multitude of complications. Increasing evidence shows that the dietary supplement with betaine, a natural chemical molecule, can effectively reduce the fat accumulation in the liver. Translational regulation is considered to play a vital role in gene expression, but whether betaine functions through the regulation of gene translational level is still unclear. To this end, RNC-seq (mRNAs bound to ribosome-nascent chain complex sequencing) and RNA-seq co-analyses were performed to identify betaine target genes by using the liver samples from high-fat diet adding betaine treated and high-fat diet treated mice. The results showed that betaine does play a lipid-lowering role by regulating the expression of gene translation levels; some NAFLD- and lipid metabolism-associated genes were differentially expressed at translational level, for example. And the translation ratio (TR) of gene significantly increased after betaine treatment. Finally, we identified a novel function gene, Gpc1, which may mediate the lipid-lowering effect of betaine in the liver. To sum up, this study depicted the molecular portrait of mice liver with or without betaine treatment from the angel of translatome and transcriptome, giving insights into the molecular mechanism of betaine-mediated lipid-lowering effect and also providing new clues for understanding and prevention of NAFLD.
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