根际
斯氏假单胞菌
根际细菌
微生物菌剂
生物
代谢组学
生物膜
假单胞菌
植物
微生物群
铁载体
细菌
遗传学
生物信息学
作者
Xinli Sun,Zhihui Xu,Jiyu Xie,Viktor H. Thomsen,Taimeng Tan,Mikael Lenz Strube,Anna Dragoš,Qirong Shen,Ruifu Zhang,Ákos T. Kovács
标识
DOI:10.1101/2021.06.02.446779
摘要
Abstract Trophic interactions play a central role in driving microbial community assembly and function. In gut or soil ecosystems, successful inoculants are always facilitated by efficient colonization, however, the metabolite exchanges between inoculants and resident bacteria are rarely studied, particularly in the rhizosphere. Here, we used bioinformatic, genetic, transcriptomic and metabonomic analyses to uncover syntrophic cooperation between inoculant ( Bacillus velezensis SQR9) and plant-beneficial indigenous Pseudomonas stutzeri in the cucumber rhizosphere. We found that the synergistic interaction of these two species is highly environmental dependent, the emergence of syntrophic cooperation was only evident in a static nutrient-rich niche, such as pellicle biofilm in addition to the rhizosphere. Our results identified branched-chain amino acids (BCAAs) biosynthesis pathway involved in syntrophic cooperation when forming coculture biofilms. Assaying the metabolome further demonstrated metabolic facilitation among the bacterial strains. In addition, biofilm matrix components from Bacillus were essential for the interaction. Importantly, the two-species consortium promoted plant growth and helped plants alleviate salt stress. In summary, we propose a mechanism in which synergic interactions between a biocontrol bacterium and a partner species promote plant health.
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