脂肪生成
油红O
生物
小RNA
免疫染色
转录组
内分泌学
内科学
信使核糖核酸
甘油三酯
脂滴
荧光素酶
脂肪组织
细胞生物学
基因表达
免疫组织化学
基因
生物化学
免疫学
转染
胆固醇
医学
作者
Yingying Zhang,Yaning Wang,Hongbao Wang,Xueyao Ma,Linsen Zan
标识
DOI:10.1016/j.mcp.2019.01.005
摘要
In this study, the potential functions of miR-224 in regulating adipogenic differentiation were explored in bovine preadipocytes. Comparative transcriptome analysis between castrated male cattle with increased intramuscular fat (IMF) and intact male cattle revealed that miR-224 and LPL were abnormally expressed, correlating negatively, and LPL was a predicted target of miR-224. A dual luciferase reporter assay confirmed the negative targeting regulatory relationship between miR-224 and LPL. When miR-224 was either overexpressed or silenced, qRT-PCR showed a negative regulatory effect on LPL. mRNA expression levels of the fat-formation-related biomarkers C/EBPα, C/EBPβ, PPARγ, FASN and PLIN1 decreased when miR-224 was overexpressed, while the opposite effect occurred and adipogenic differentiation followed when miR-224 was inhibited. Oil Red O staining. Triglyceride (TG) levels and immunostaining revealed that the accumulation of lipid droplets decreased or increased accordingly. Taken together, our data demonstrated that miR-224 regulated the adipogenic differentiation of bovine preadipocytes by targeting LPL. This provides insight into the molecular basis of IMF deposition in beef cattle.
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