突变
大肠杆菌
重组DNA
生物
核糖体
核糖体结合位点
定点突变
计算生物学
蛋白质工程
分子生物学
肽库
基因组文库
表达式向量
翻译(生物学)
遗传学
突变
基因
肽序列
生物化学
酶
信使核糖核酸
突变体
核糖核酸
作者
B. J. Wilson,Curtis Kautzer,Douglas Antelman
出处
期刊:PubMed
日期:1994-11-01
卷期号:17 (5): 944-53
被引量:7
摘要
This report describes a method whereby library mutagenesis combined with drug selection was used to generate unique and efficient ribosome-binding sites (RBS) for expressing recombinant proteins in Escherichia coli. The RBS was deleted from a vector expressing beta-lactamase and replaced with a 16-base sequence containing a library of mutations. Selection of the library with ampicillin yielded several unique RBS sequences that were more efficient than ompA RBS for expressing a bacterial (beta-lactamase) and a mammalian protein (single-chain Fv antibody). The described approach provides a practical means to improve recombinant protein expression and, also, provides new sequences to further evaluate the complex regulatory mechanism underlying translation initiation.
科研通智能强力驱动
Strongly Powered by AbleSci AI