端粒酶
端粒酶逆转录酶
细胞凋亡
流式细胞术
A549电池
血细胞仪
细胞培养
生长抑制
化学
分子生物学
生物
细胞生物学
癌症研究
生物化学
基因
遗传学
作者
Dong Il Park,Jae Hun Lee,Sung‐Kwon Moon,Cheorl-Ho Kim,Yong Tae Lee,JaeHun Cheong,Byung Tae Choi,Yung Hyun Choi
标识
DOI:10.1016/j.phrs.2004.11.003
摘要
The objective of the present study was to investigate the effect of aqueous extract from the root of Platycodon grandiflorum (AEPG) on the cell growth and apoptosis in human lung carcinoma cell line A549. Exposure of A549 cells to AEPG resulted in growth inhibition and induction of apoptosis in a dose-dependent manner as measured by hemocytometer counts, fluorescence microscopy and flow cytometry analysis. This increase in apoptosis was associated with a decrease in Bcl-2 expression, an increase of Bax and an activation of caspase-3. AEPG treatment markedly inhibited the activity of telomerase in a dose-dependent fashion. Additionally, the expression of human telomerase reverse transcriptase (hTERT), a main determinant of the telomerase enzymatic activity, was progressively down-regulated by AEPG treatment. These findings suggest that the apoptotic events by AEPG were associated with the diminished telomerase activity and down-regulation of Bcl-2 expression.
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