Zeta电位
化学
胶体
表面电荷
微泡
离子强度
小泡
磷酸盐
生物物理学
磷酸盐缓冲盐水
细胞外小泡
细胞外
色谱法
化学工程
纳米颗粒
生物化学
膜
水溶液
有机化学
细胞生物学
基因
工程类
生物
小RNA
物理化学
作者
Getnet Midekessa,Kasun Godakumara,James Ord,Janeli Viil,Freddy Lättekivi,Keerthie Dissanayake,Sergei Kopanchuk,Ago Rinken,Aneta Andronowska,Sourav Bhattacharjee,Toonika Rinken,Alireza Fazeli
出处
期刊:ACS omega
[American Chemical Society]
日期:2020-06-30
卷期号:5 (27): 16701-16710
被引量:297
标识
DOI:10.1021/acsomega.0c01582
摘要
Extracellular vesicles (EVs), including exosomes and microvesicles (<200 nm), play a vital role in intercellular communication and carry a net negative surface charge under physiological conditions. Zeta potential (ZP) is a popular method to measure the surface potential of EVs, while used as an indicator of surface charge, and colloidal stability influenced by surface chemistry, bioconjugation, and the theoretical model applied. Here, we investigated the effects of such factors on ZP of well-characterized EVs derived from the human choriocarcinoma JAr cells. The EVs were suspended in phosphate-buffered saline (PBS) of various phosphate ionic concentrations (0.01, 0.1, and 1 mM), with or without detergent (Tween-20), or in the presence (10 mM) of different salts (NaCl, KCl, CaCl2, and AlCl3) and at different pH values (4, 7, and 10) while the ZP was measured. The ZP changed inversely with the buffer concentration, while Tween-20 caused a significant (p < 0.05) lowering of the ZP. Moreover, the ZP was significantly (p < 0.05) less negative in the presence of ions with higher valency (Al3+/Ca2+) than in the presence of monovalent ones (Na+/K+). Besides, the ZP of EVs became less negative at acidic pH, and vice versa. The integrated data underpins the crucial role of physicochemical attributes that influence the colloidal stability of EVs.
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