已入深夜,您辛苦了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!祝你早点完成任务,早点休息,好梦!

Ethanol extracts of Sanguisorba officinalis L. suppress TNF-α/IFN-γ-induced pro-inflammatory chemokine production in HaCaT cells

哈卡特 趋化因子 肿瘤坏死因子α 化学 分子生物学 免疫学 生物 炎症 生物化学 体外
作者
Ju-Hye Yang,Youn–Hwan Hwang,Min-Jung Gu,Won‐Kyung Cho,Jin Yeul
出处
期刊:Phytomedicine [Elsevier]
卷期号:22 (14): 1262-1268 被引量:62
标识
DOI:10.1016/j.phymed.2015.09.006
摘要

Sanguisorba officinalis L. (SOL) is a perennial plant widely distributed in Asia, its roots are well-known as a traditional herbal medicine to treat burns, chronic intestinal infections, scalds, and inflammation in Korea. Also, the roots of SOL are used for treatment of many types of allergic skin diseases, including urticarial, eczema, and allergic dermatitis.In this study we investigated the underlying mechanism of anti-inflammatory effect of an ethanol extract of SOL roots (ESOL).The ability of ESOL to inhibit inflammatory skin disorder was tested in human keratinocyte HaCaT cells.Viability test using MTT assay were used to determine non-cytotoxic concentrations of ESOL on HaCaT cells. ESOL-mediated inhibition of the tumor necrosis factor (TNF)-α/interferon (IFN)-γ-induced production of pro-inflammatory chemokines-such as macrophage-derived chemokine (MDC), regulated on activation, normal T-cell expressed and secreted (RANTES), interleukin (IL)-8, and thymus and activation regulated chemokine (TARC)-at the mRNA level was determined by real-time reverse transcription-polymerase chain reaction (RT-PCR). The ability of ESOL to reduce the expression of pro-inflammatory marker proteins was evaluated by Western blot analysis and immunocytochemistry.ESOL reduced the production of MDC, RANTES, IL-8, and TARC in HaCaT cells stimulated with TNF-α/IFN-γ at both protein and mRNA levels. ESOL also suppressed the phosphorylation of signal transducer and activator of transcription (STAT)-1, extracellular signal-regulated kinase (ERK), and inhibited both nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor-alpha (IκB-α) degradation and the nuclear translocation of NF-κB/p65. ESOL exerts anti-inflammatory effects by suppressing the expression of TNF-α/IFN-γ-stimulated chemokines and pro-inflammatory molecules via a blockade NF-κB, STAT-1, and ERK activation.Our results suggest the preventive potential of ESOL as a herbal medicine for the treatment of inflammatory skin diseases.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
__完成签到 ,获得积分20
刚刚
2秒前
练习者发布了新的文献求助10
3秒前
5秒前
杉进完成签到 ,获得积分10
10秒前
Cheny发布了新的文献求助10
10秒前
bukeshuo发布了新的文献求助10
15秒前
17秒前
21秒前
宰宰小熊完成签到 ,获得积分20
23秒前
宇文傲龙完成签到 ,获得积分10
25秒前
懵懂的半蕾完成签到 ,获得积分10
29秒前
29秒前
orixero应助lele7458采纳,获得10
36秒前
Dara完成签到,获得积分10
38秒前
39秒前
小二郎应助RW采纳,获得10
40秒前
羊羊羊完成签到,获得积分10
42秒前
勤恳青槐完成签到 ,获得积分10
43秒前
ISLAND完成签到,获得积分10
46秒前
糖加三勺完成签到 ,获得积分10
47秒前
52秒前
菜菜完成签到 ,获得积分10
1分钟前
1分钟前
1分钟前
共享精神应助科研通管家采纳,获得10
1分钟前
1分钟前
大模型应助科研通管家采纳,获得10
1分钟前
1分钟前
1分钟前
1分钟前
1分钟前
1分钟前
寻道图强应助科研通管家采纳,获得30
1分钟前
1分钟前
kacey发布了新的文献求助10
1分钟前
花花懿懿完成签到,获得积分10
1分钟前
lix完成签到 ,获得积分10
1分钟前
kacey完成签到,获得积分20
1分钟前
花花懿懿发布了新的文献求助30
1分钟前
高分求助中
Evolution 10000
ISSN 2159-8274 EISSN 2159-8290 1000
Becoming: An Introduction to Jung's Concept of Individuation 600
Ore genesis in the Zambian Copperbelt with particular reference to the northern sector of the Chambishi basin 500
A new species of Coccus (Homoptera: Coccoidea) from Malawi 500
A new species of Velataspis (Hemiptera Coccoidea Diaspididae) from tea in Assam 500
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3162246
求助须知:如何正确求助?哪些是违规求助? 2813263
关于积分的说明 7899489
捐赠科研通 2472504
什么是DOI,文献DOI怎么找? 1316446
科研通“疑难数据库(出版商)”最低求助积分说明 631317
版权声明 602142