Zinc Transporters ZnT1 (Slc30a1), Zip8 (Slc39a8), and Zip10 (Slc39a10) in Mouse Red Blood Cells Are Differentially Regulated during Erythroid Development and by Dietary Zinc Deficiency

运输机 DMT1型 缺锌(植物性疾病) 生物化学 化学 红细胞 细胞内 生物 基因 有机化学
作者
Moon‐Suhn Ryu,Louis A. Lichten,Juan P. Liuzzi,Robert J. Cousins
出处
期刊:Journal of Nutrition [Oxford University Press]
卷期号:138 (11): 2076-2083 被引量:83
标识
DOI:10.3945/jn.108.093575
摘要

Zinc is essential for normal erythroid cell functions and therefore intracellular zinc homeostasis during erythroid differentiation is tightly regulated. However, a characterization of zinc transporters in erythrocytes has not been conducted. The membrane fraction of mature mouse RBC was screened for zinc transporter expression using western analysis as a first step in the characterization process. ZnT1, Zip8, and Zip10 were detected among the 12 transporter proteins tested. We examined expression of these zinc transporters during erythropoietin (EPO)-induced differentiation of splenic erythroid progenitor cells into reticulocytes. Both Zip8 and Zip10 mRNA increased by 2-6 h after addition of EPO to the cells. In contrast, maximal RNA levels for the zinc transporter ZnT1 and erythroid delta-aminolevulinic acid synthase were only produced by 24 h after EPO. We confirmed these changes in transcript abundance by western analysis. Dietary zinc status influences zinc-dependent functions of RBC. To determine whether the identified zinc transporters respond to dietary zinc status, mice were fed a zinc-deficient or control diet. Incorporation of (65)Zn into erythrocytes in vitro was significantly increased in cells from the zinc-deficient mice. Western analysis and densitometry revealed that erythrocyte Zip10 was upregulated and ZnT1 was downregulated in the zinc-depleted mice. Zip8 was not affected by restricted zinc intake. Collectively, these data suggest that the zinc transporters ZnT1, Zip8, and Zip10 are important for zinc homeostasis in erythrocytes and that ZnT1 and Zip10 respond to the dietary zinc supply.

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
baixue发布了新的文献求助10
刚刚
2秒前
3秒前
房靳发布了新的文献求助10
4秒前
学术机器1发布了新的文献求助10
4秒前
优雅的化蛹完成签到,获得积分10
6秒前
Minicoper完成签到,获得积分10
6秒前
冷酷思远发布了新的文献求助10
7秒前
TJC发布了新的文献求助10
8秒前
9秒前
10秒前
宝川发布了新的文献求助10
11秒前
小王发布了新的文献求助10
11秒前
13秒前
wo_qq111完成签到 ,获得积分10
13秒前
13秒前
13秒前
惠JUI完成签到,获得积分10
14秒前
15秒前
16秒前
脑洞疼应助桑葚啊采纳,获得10
16秒前
17秒前
17秒前
香蕉觅云应助鳗鱼嫣然采纳,获得20
18秒前
tang发布了新的文献求助30
19秒前
Minicoper发布了新的文献求助10
19秒前
19秒前
喆喆发布了新的文献求助10
19秒前
19秒前
20秒前
20秒前
轩贝完成签到,获得积分10
22秒前
研友_浑不斜完成签到,获得积分10
22秒前
勤恳的元绿完成签到 ,获得积分10
23秒前
宝川完成签到,获得积分10
23秒前
Lynk369发布了新的文献求助10
23秒前
心流发布了新的文献求助10
23秒前
聂宇航发布了新的文献求助30
24秒前
贰陆发布了新的文献求助10
24秒前
高分求助中
中国国际图书贸易总公司40周年纪念文集 大事记1949-1987 2000
TM 5-855-1(Fundamentals of protective design for conventional weapons) 1000
草地生态学 880
Threaded Harmony: A Sustainable Approach to Fashion 799
Queer Politics in Times of New Authoritarianisms: Popular Culture in South Asia 500
Livre et militantisme : La Cité éditeur 1958-1967 500
Retention of title in secured transactions law from a creditor's perspective: A comparative analysis of selected (non-)functional approaches 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3058079
求助须知:如何正确求助?哪些是违规求助? 2714263
关于积分的说明 7439873
捐赠科研通 2359489
什么是DOI,文献DOI怎么找? 1250095
科研通“疑难数据库(出版商)”最低求助积分说明 607383
版权声明 596392