电介质
介电泳
电容
信号(编程语言)
化学
电容式探头
电极
扭矩
分子物理学
材料科学
分析化学(期刊)
光电子学
物理
色谱法
热力学
计算机科学
物理化学
程序设计语言
作者
Song‐I Han,Young‐Don Joo,Ki-Ho Han
出处
期刊:Analyst
[Royal Society of Chemistry]
日期:2013-01-01
卷期号:138 (5): 1529-1529
被引量:134
摘要
This paper presents an effective electrorotation technique for measuring the dielectric properties of cells using a superposed electrical signal, which can simultaneously generate negative quadrupolar dielectrophoretic (nQDEP) force and electrorotational (ROT) torque. The proposed technique involves a three-dimensional (3D) octode, which includes four electrodes arranged in a crisscross pattern on the top and bottom of a microchannel, respectively. A single cell was trapped in the center of the 3D octode by the nQDEP force and simultaneously rotated by the ROT torque. Using the proposed electrorotation technique, ROT spectra of human leukocyte subpopulations (T and B lymphocytes, granulocytes, and monocytes) and metastatic human breast (SkBr3) and lung (A549) cancer cell lines were accurately measured without any disturbance. Torque on the cells generated by the ROT signal was analyzed theoretically based on the single-shell dielectric model for the cells. Furthermore, the dielectric properties of the cells, such as area-specific membrane capacitance and cytoplasm conductivity, were extracted using the measured ROT spectra and the analyzed torque.
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