生物
DNA甲基化
DNMT1型
DNA甲基转移酶
体育锻炼的表观遗传学
DNA去甲基化
CpG站点
甲基转移酶
基因表达调控
甲基化
细胞生物学
DNA
遗传学
分子生物学
基因表达
基因
作者
Jesús Espada,Héctor Peinado,Lidia Lopez-Serra,Fernando Setien,Paula Lopez-Serra,Anna Portela,Jaime Renart,Elisa Carrasco,Maria L. Calvo,Angeles Juarranz,Amparo Cano,Manel Esteller
摘要
Mammalian DNA methyltransferase 1 (DNMT1) is essential for maintaining DNA methylation patterns after cell division. Disruption of DNMT1 catalytic activity results in whole genome cytosine demethylation of CpG dinucleotides, promoting severe dysfunctions in somatic cells and during embryonic development. While these observations indicate that DNMT1-dependent DNA methylation is required for proper cell function, the possibility that DNMT1 has a role independent of its catalytic activity is a matter of controversy. Here, we provide evidence that DNMT1 can support cell functions that do not require the C-terminal catalytic domain. We report that PCNA and DMAP1 domains in the N-terminal region of DNMT1 are sufficient to modulate E-cadherin expression in the absence of noticeable changes in DNA methylation patterns in the gene promoters involved. Changes in E-cadherin expression are directly associated with regulation of β-catenin-dependent transcription. Present evidence suggests that the DNMT1 acts on E-cadherin expression through its direct interaction with the E-cadherin transcriptional repressor SNAIL1.
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