质谱法
质谱成像
代谢组学
化学
环境电离
分析化学(期刊)
解吸电喷雾电离
解吸
激光器
原位
样品制备
代谢物分析
代谢物
电离
色谱法
化学电离
光学
离子
生物化学
物理
吸附
有机化学
作者
Xue Xiao,Xiaokang Guan,Zhouyi Xu,Qiao Lu
出处
期刊:Metabolites
[Multidisciplinary Digital Publishing Institute]
日期:2024-02-21
卷期号:14 (3): 131-131
被引量:1
标识
DOI:10.3390/metabo14030131
摘要
With its high resolving power and sensitivity, mass spectrometry is considered the most informative technique for metabolite qualitation and quantification in the plant sciences. However, the spatial location information, which is crucial for the exploration of plant physiological mechanisms, is lost. Mass spectrometry imaging (MSI) is able to visualize the spatial distribution of a large number of metabolites from the complex sample surface in a single experiment. In this paper, a flexible and low-cost laser desorption–dielectric barrier discharge ionization-MSI (LD-DBDI-MSI) platform was constructed by combining an LD system with an in-line DBDI source, a high-precision sample translation stage, and an ambient mass spectrometer. It can be operated at a spatial resolution of 20 μm in an atmospheric environment and requires minimal sample preparation. This study presents images of in-situ metabolic profiling of two kinds of plants from different origins, a wild and a farmed Rheum palmatum L. From the screen of these two root sections, the wild one presented five more endogenous molecules than the farmed one, which provides information about the differences in metabolomics.
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