医学
动脉瘤
动脉瘤
升主动脉
主动脉瘤
降主动脉
胸主动脉
队列
心脏病学
主动脉
放射科
内科学
作者
Malak Elbatarny,Uroš Kuzmanov,Daniella Eliathamby,Vivian H. Chu,Rashmi Nedadur,Cristine J. Reitz,Omar Hamed,Craig A. Simmons,Jennifer Chung,Bo Wang,Maral Ouzounian,Anthony O. Gramolini
出处
期刊:Circulation
[Ovid Technologies (Wolters Kluwer)]
日期:2023-11-07
卷期号:148 (Suppl_1)
标识
DOI:10.1161/circ.148.suppl_1.13601
摘要
Background: Acute Type A Aortic Dissection (ATAD) is a surgical emergency with 18% mortality. Anatomic segment (root, ascending, arch, descending) impacts aneurysm natural history but mechanisms remain unclear. Aim: To compare proteomic profiles of human thoracic aortic segments that could account for distinct phenotypes and clinical outcomes; to analyze the largest cohort to date with enhanced depth of coverage. Methods: Aortic tissues were collected (N=148) from 82 unique individuals and analyzed using our customized proteomics protocol ( Figure 1A ). Conventional statistics, machine learning (t-distributed Stochastic Neighbour Embedding, t-SNE), and functional enrichment analyses were used to characterize significant phenotypic differences by segment. Differential protein expression was validated using immunofluorescence. Results: From all samples, 7251 proteins were identified (5660 quantified), exceeding literature (by 100s-1000s). Significant differences were greatest in comparisons of root, ascending, and descending aorta ( Figure 1B-C ). Root vs descending and ascending vs descending comparisons had clear separation in t-SNE analysis; root and ascending samples also clustered modestly ( Figure 1D ). MFAP4, a cellular-binding protein previously associated with descending thoracic dissection in Marfan patients, was significantly elevated in ascending aortic segments compared to root and descending. This was validated by immunofluorescence ( Figure 1E ). Conclusions: We extensively profiled thoracic aneurysm tissue using enhanced coverage, customized proteomics from the largest known cohort of human samples. Thoracic aneurysm phenotype differs by aortic segment as a function of intrinsic biochemical (proteomic) processes. Potential thoracic aneurysm biomarkers likely must account for aortic segment. Multiomic integrative analysis (DNA, RNA, post-translational data) are underway.
科研通智能强力驱动
Strongly Powered by AbleSci AI