查尔酮合酶
黄烷酮
聚酮合酶
生物合成
查尔酮异构酶
查尔酮
化学
类黄酮生物合成
类黄酮
基因簇
聚酮
非核糖体肽
ATP合酶
立体化学
生物化学
酶
生物
基因
转录组
基因表达
抗氧化剂
作者
Wei Zhang,Xuan Zhang,Dandan Feng,Yajing Liang,Zhenying Wu,Siyu Du,Yu Zhou,Ce Geng,Ping Men,Chunxiang Fu,Xiaowan Huang,Xuefeng Lü
标识
DOI:10.1002/ange.202215529
摘要
Abstract Flavonoids are important plant natural products with variable structures and bioactivities. All known plant flavonoids are generated under the catalysis of a type III polyketide synthase (PKS) followed by a chalcone isomerase (CHI) and a flavone synthase (FNS). In this study, the biosynthetic gene cluster of chlorflavonin, a fungal flavonoid with acetolactate synthase inhibitory activity, was discovered using a self‐resistance‐gene‐directed strategy. A novel flavonoid biosynthetic pathway in fungi was revealed. A core nonribosomal peptide synthetase‐polyketide synthase (NRPS‐PKS) is responsible for the generation of the key precursor chalcone. Then, a new type of CHI catalyzes the conversion of a chalcone into a flavanone by a histidine‐mediated oxa‐Michael addition mechanism. Finally, the desaturation of flavanone to flavone is catalyzed by a new type of FNS, a flavin mononucleotide (FMN)‐dependent oxidoreductase.
科研通智能强力驱动
Strongly Powered by AbleSci AI