Protein-Assisted Molybdenum Disulfide as Biomimetic Nanozyme for Antibacterial Application

Molybdenum-based nanomaterials with variable oxidation states can be developed as nanozyme catalysts. In this work, we developed a one-pot method for the preparation of molybdenum disulfide assisted by protein. Protamine was used as a cationic template to link molybdate anions and form complexes. During hydrothermal synthesis, protamine can affect the nucleation process of molybdenum disulfide and inhibit their aggregation, which facilitates the fabrication of small-sized molybdenum disulfide nanoparticles. Moreover, the abundant amino/guanidyl groups of protamine could both physically adsorb and chemically bond to molybdenum disulfide and further modulate the crystal structures. The optimized size and crystalline structure enabled a higher exposure of active sites, which enhanced the peroxidase-like activity of molybdenum disulfide/protamine nanocomposites. Meanwhile, the antibacterial activity of protamine was retained in the molybdenum disulfide/protamine nanocomposites, which could synergize with the peroxidase-like activity of molybdenum disulfide to kill bacteria. Therefore, the molybdenum disulfide/protamine nanocomposites are good candidates for antibacterial agents with lower chances of antimicrobial resistance. This study establishes an easy way to design artificial nanozymes by compounding suitable components.