Electrochemiluminescence Sensor with Controlled-Release Triggering Electrostatic Attraction Elimination Mechanism for Trenbolone Trace Detection

化学 生物传感器 电化学发光 检出限 电极 基质(水族馆) 氧化还原 纳米技术 组合化学 色谱法 无机化学 生物化学 海洋学 地质学 物理化学 材料科学
作者
Lu Zhao,Xiang Ren,Hongmin Ma,Huan Wang,Yuyang Li,Qin Wei,Dan Wu,Huangxian Ju
出处
期刊:Analytical Chemistry [American Chemical Society]
卷期号:95 (36): 13463-13469 被引量:6
标识
DOI:10.1021/acs.analchem.3c01359
摘要

A controlled-release strategy can meet the needs of sensitive environmental monitoring for pollutants through a self-on/off mode. In this work, an electrochemiluminescence (ECL) biosensor with controlled-release triggering electrostatic attraction elimination and biomolecular stimulated response strategies was constructed to detect environmental steroid hormones sensitively. The blocked pores on the aminated mesoporous silica nanocontainers were opened by specific binding between the trenbolone (TB) antigen and the antibody. The released l-cysteine counteracted the negative charge on the MnO2 NF surface through the redox reaction between –SH and MnO2, making the electrostatic interaction between the MnO2 NFs and the Ru(dcbpy)32+ disappear. Ru(dcbpy)32+ released an ECL signal on the electrode, thus completing the controlled-release triggering electrostatic attraction elimination strategy. In addition, with the TB antibody as the target and the competition strategy between the TB antigen and the standard substance, the constructed controlled-release ECL biosensor was used to detect the TB standard substance. Moreover, MnO2 NFs as the substrate of the ECL biosensor increased the active specific surface area of the electrode, effectively catalyzing the production of OH• and O2•–, thus endowing the ECL biosensor with coreactant-catalytic enhancement characteristic and further improving its ECL performance. This sensitive signal response brought about a low limit of detection of 2.53 fg/mL for the constructed ECL biosensor, which contributed a feasible idea for efficient trace analysis of pollutants in the environment.
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