Not all cytochrome b5s are created equal: how a specific CytB5 boosts forskolin biosynthesis in Saccharomyces cerevisiae

酿酒酵母 福斯科林 生物合成 生物化学 生物 酵母 受体
作者
Victor Forman,Dan Luo,Sotirios C. Kampranis,Dan Stærk,Birger Lindberg Møller,Irini Pateraki
出处
期刊:Metabolic Engineering [Elsevier BV]
标识
DOI:10.1016/j.ymben.2024.10.008
摘要

Cytochrome B5s, or CytB5s, are small heme-binding proteins, ubiquitous across all kingdoms of life that serve mainly as electron donors to enzymes engaged in oxidative reactions. They often function as redox partners of the cytochrome P450s (CYPs), a superfamily of enzymes participating in multiple biochemical processes. In plants, CYPs catalyze key reactions in the biosynthesis of plant specialized metabolites with their activity dependent on electron donation often from cytochrome P450 oxidoreductases (CPRs or PORs). In eukaryotic microsomal CYPs, CytB5s frequently participate in the electron transfer process although their exact role remains understudied, especially in plant systems. In this study, we assess the role of CytB5s in the heterologous biotechnological production of plant specialized metabolites in yeast. For this, we used as a case-study the biosynthesis of forskolin - a bioactive diterpenoid produced exclusively from the plant Coleus forskohlii. The complete biosynthetic pathway for forskolin is known and includes three CYP enzymes. We reconstructed the entire forskolin pathway in the yeast Saccharomyces cerevisiae, and upon co-expression of the three CytB5s - identified in C. forskohlii transcriptomes - alleviation of a CYP-related bottleneck step was noticed only when a specific CytB5, CfCytB5A, was used. Co-expression of CfCytB5A in yeast, in combination with forskolin pathway engineering, resulted in forskolin production at titers of 1.81 g/L in a bioreactor. Our findings demonstrate that CytB5s not only play an important role in plant specialized metabolism but also, they can interact with precision with specific CYPs, indicating that the properties of CytB5s are far from understood. Moreover, our work highlights how CytB5s may act as indispensable components in the sustainable microbial production of plant metabolites, when their biosynthetic pathways involve CYP enzymes.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
Menta1y完成签到,获得积分10
刚刚
czzlancer完成签到,获得积分10
1秒前
汶溢完成签到,获得积分10
1秒前
xsss完成签到,获得积分10
2秒前
TAN完成签到,获得积分10
2秒前
通通通发布了新的文献求助10
3秒前
liudw完成签到,获得积分10
3秒前
丹丹子完成签到 ,获得积分10
4秒前
时光完成签到,获得积分10
4秒前
5秒前
充电宝应助vsvsgo采纳,获得10
7秒前
123完成签到 ,获得积分10
9秒前
Ammr完成签到 ,获得积分10
9秒前
无限的依波完成签到,获得积分10
9秒前
姽婳wy发布了新的文献求助10
10秒前
lemon完成签到,获得积分10
10秒前
传奇3应助duckspy采纳,获得30
11秒前
陈木木完成签到,获得积分10
12秒前
可可西里完成签到,获得积分10
13秒前
奋斗蜗牛完成签到,获得积分10
13秒前
CipherSage应助眼睛大的擎苍采纳,获得10
13秒前
打打应助小小酥采纳,获得10
14秒前
fox完成签到 ,获得积分10
14秒前
僦是卜够完成签到 ,获得积分10
15秒前
小马甲应助嘉梦采纳,获得10
18秒前
qiqi完成签到,获得积分10
19秒前
19秒前
科研乞丐应助Jerry采纳,获得20
20秒前
vsvsgo发布了新的文献求助10
22秒前
Jeffrey完成签到,获得积分10
23秒前
明理采珊完成签到,获得积分10
23秒前
lll发布了新的文献求助10
23秒前
vsvsgo发布了新的文献求助10
26秒前
慎之完成签到 ,获得积分10
26秒前
我是微风完成签到,获得积分10
26秒前
传奇3应助木子采纳,获得30
28秒前
vsvsgo发布了新的文献求助10
30秒前
feitian201861完成签到,获得积分10
30秒前
32秒前
高分求助中
【提示信息,请勿应助】关于scihub 10000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 3000
徐淮辽南地区新元古代叠层石及生物地层 3000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Handbook of Industrial Diamonds.Vol2 1100
Global Eyelash Assessment scale (GEA) 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 550
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4038235
求助须知:如何正确求助?哪些是违规求助? 3575992
关于积分的说明 11374009
捐赠科研通 3305760
什么是DOI,文献DOI怎么找? 1819276
邀请新用户注册赠送积分活动 892662
科研通“疑难数据库(出版商)”最低求助积分说明 815022