噬菌体展示
噬菌体
单克隆抗体
抗体
免疫球蛋白Fab片段
肽库
生物
分子生物学
计算生物学
遗传学
肽序列
基因
互补决定区
噬菌体
大肠杆菌
作者
Haiyong Peng,Christoph Rader
出处
期刊:CSH Protocols
[Cold Spring Harbor Laboratory]
日期:2024-10-15
标识
DOI:10.1101/pdb.prot108597
摘要
Phage display is a powerful method for the de novo generation and affinity maturation of human monoclonal antibodies from naive, immune, and synthetic antibody repertoires. The pComb3 phagemid family of phage display vectors facilitates the selection of human monoclonal antibody libraries in the monovalent Fab format, which consists of human variable domains V H and V L (V κ or V λ ), fused to the human constant domains C H 1 of IgG1 and C L (C κ or C λ ), respectively. Here, we describe the use of a pComb3 derivative, phagemid pC3C, for the generation of human Fab libraries with randomly combined human variable domains (V H , V κ , and V λ ) of high sequence diversity, starting from the preparation of mononuclear cells from blood and bone marrow. Depending on the complexity of the parental antibody repertoire, the protocol can be scaled for yielding a library size of 10 8 –10 11 independent human Fab clones. As such, it can be used, for instance, for the generation of a large naive human Fab library from healthy individuals or for the generation of a specialized immune human Fab library from individuals with an endogenous antibody response of interest.
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