环介导等温扩增
逆转录环介导等温扩增
化学
检出限
软件可移植性
色谱法
荧光染料
逆转录酶
计算生物学
DNA
分子生物学
核糖核酸
聚合酶链反应
计算机科学
基因
生物化学
生物
程序设计语言
作者
Manpreet Kaur,Khadijah Ayarnah,Danielle Duanis‐Assaf,Noam Alkan,Evgeni Eltzov
标识
DOI:10.1016/j.aca.2023.341394
摘要
Paper-based analytical devices (PADs) have gained enormous attention because of their low-cost, simple fabrication, and portability. Here, we propose a paper-based device for performing reverse transcription loop-mediated isothermal amplification (RT-LAMP) with real-time simultaneous detection of C. gloeosporioides latent infections in tomatoes. RT-LAMP-based PAD platform comprises a paper substrate on which the DNA amplification reaction occurs. Among different types of tested papers, cellulose membrane (grade 4) enabled effective visualization of the amplification result. The assay was found highly selective for the latent stage of C. gloeosporioides with lower limit of detection (LOD) of 0.5 pg of total extracted RNA. The developed assay generated the results within 40 min and hence can be efficiently employed for identifying C. gloeosporioides in resource-limited settings.
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