微管
细胞生物学
生物
生长锥
微管相关蛋白
τ蛋白
神经科学
动力学(音乐)
生物物理学
心理学
轴突
内科学
医学
教育学
疾病
阿尔茨海默病
作者
Xiaohuan Sun,Wenqian Yu,Peter W. Baas,Kazuhito Toyo‐oka,Liang Qiang
标识
DOI:10.1101/2024.02.21.581458
摘要
Abstract Association of tau with microtubules causes them to be labile while association of MAP6 with microtubules causes them to be stable. As axons differentiate and grow long, tau and MAP6 segregate from one another on individual microtubules, resulting in the formation of stable and labile domains. The functional significance of the yin/yang relationship between tau and MAP6 remained speculative in those studies, with one idea being that such a relationship assists in balancing morphological stability with plasticity. Here, using primary rodent neuronal cultures, we show that depletion of tau has opposite effects compared to depletion of MAP6 on the rate of neuronal development, the efficiency of growth cone turning, and the number of processes and axonal branches. Opposite effects to those of tau depletion were also observed on the rate of neuronal migration, in an in vivo assay, when we depleted MAP6. When tau and MAP6 were depleted together in the cell culture assays, the morphological phenotypes negated one another. Tau and MAP6 are multifunctional proteins, but the present results suggest that the observed effects of their depletion on neuronal development are likely due to their opposite roles in regulating microtubule dynamics. Summary Tau and MAP6 play antagonistic roles in regulating multiple aspects of neuronal development, presumably via their antagonistic effects on microtubule dynamics.
科研通智能强力驱动
Strongly Powered by AbleSci AI