Integration of Immunosyringe Sensors with Bar-Chart Chips for Prick-and-Read Testing of Prostate Specific Antigen

Pressure-based signal transduction is of promise in developing microfluidic immunoassays such as volumetric bar-chart chips (V-chips), but new working principles are required to further simplify the methods in point-of-care testing (POCT). Herein, we developed immunosyringe sensors and integrated them with bar-chart chips for simple prick-and-read testing of prostate specific antigen (PSA) as a model target. Disposable syringes served as the host for the construction of the sandwich-type immuno-recognition system. Platinum nanoparticles (Pt NPs) as the peroxidase-mimicking detection probe catalyzed the decomposition of H