微分脉冲伏安法
检出限
唾液
严重急性呼吸综合征冠状病毒2型(SARS-CoV-2)
生物传感器
冠状病毒
聚(N-异丙基丙烯酰胺)
2019年冠状病毒病(COVID-19)
电化学
电化学气体传感器
材料科学
线性范围
纳米技术
化学
循环伏安法
病毒学
色谱法
共聚物
医学
生物化学
电极
传染病(医学专业)
疾病
病理
聚合物
物理化学
复合材料
作者
Hao Chen,Zhi Yuan Hou,Die Chen,Li T,Yi Ming Wang,Marcelo A. Lima,Ying Yang,Zhenzhong Guo
出处
期刊:PubMed
日期:2023-03-20
卷期号:36 (3): 269-278
被引量:1
摘要
Late 2019 witnessed the outbreak and widespread transmission of coronavirus disease 2019 (COVID-19), a new, highly contagious disease caused by novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Consequently, considerable attention has been paid to the development of new diagnostic tools for the early detection of SARS-CoV-2.In this study, a new poly-N-isopropylacrylamide microgel-based electrochemical sensor was explored to detect the SARS-CoV-2 spike protein (S protein) in human saliva. The microgel was composed of a copolymer of N-isopropylacrylamide and acrylic acid, and gold nanoparticles were encapsulated within the microgel through facile and economical fabrication. The electrochemical performance of the sensor was evaluated through differential pulse voltammetry.Under optimal experimental conditions, the linear range of the sensor was 10 -13-10 -9 mg/mL, whereas the detection limit was 9.55 fg/mL. Furthermore, the S protein was instilled in artificial saliva as the infected human saliva model, and the sensing platform showed satisfactory detection capability.The sensing platform exhibited excellent specificity and sensitivity in detecting spike protein, indicating its potential application for the time-saving and inexpensive detection of SARS-CoV-2.
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