小泡
细菌
肺炎克雷伯菌
革兰氏阴性菌
膜
克
超离心机
细菌外膜
革兰氏阳性菌
微生物学
细胞外小泡
化学
肺炎链球菌
生物
生物化学
色谱法
大肠杆菌
细胞生物学
遗传学
基因
作者
Ana Rita Narciso,Marie‐Stéphanie Aschtgen
出处
期刊:Methods in molecular biology
日期:2023-01-01
卷期号:: 61-70
标识
DOI:10.1007/978-1-0716-3243-7_4
摘要
Membrane vesicles are produced by all Gram-negative and Gram-positive bacteria investigated so far. Membrane vesicles are spherical bilayers of phospholipids released by the bacteria to their surrounding environment and whose average size is comprised between 20 and 300 nm. The purification of these vesicles is often a challenge, as the yield and purity are often crucial for further analyses or use. In this chapter, we describe the most used method to isolate membrane vesicles from culture supernatant of Streptococcus pneumoniae and Klebsiella pneumoniae using ultracentrifugation followed by a density gradient method.
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