生物传感器
支链淀粉
检出限
分析物
阿卡波糖
膜
化学
色谱法
试剂
纳米技术
介孔二氧化硅
材料科学
介孔材料
淀粉
直链淀粉
有机化学
生物化学
酶
催化作用
作者
Binglu Zhao,Mashooq Khan,Yulin Liu,Wenjun Tai,Chongyang Mu,Wen‐Li Wu,Mei Zhao,Yaohong Ma,Li Yu,Jin‐Ming Lin,Qiongzheng Hu
标识
DOI:10.1016/j.cclet.2023.108462
摘要
Paper-based biosensors are widely employed in point-of-care testing (POCT) due to their convenience, portability, low cost, and ease of use. This study reports an integrated distance-based paper biosensor fabricated with a mesoporous membrane coated with stimuli-responsive polymer. The detection of α-amylase (AMY) using amylopectin-coated mesoporous membrane is demonstrated as an example. After introducing the AMY solution, it is observed that the aqueous solution flows along the paper strip due to AMY-catalyzed hydrolysis of amylopectin. The flow distance is proportional to the concentration of AMY with a detection limit as low as 4 mU/mL. In addition, the detection of AMY is demonstrated in human serum. Furthermore, the inhibitory effect of acarbose on AMY is evaluated. This reagent-free and disposable biosensor allows single-step rapid detection of the analyte. This approach is very promising for the development of user-friendly, equipment-free, and cost-effective biosensors with remarkable sensitivity and excellent selectivity for disease diagnosis and hypoglycemic drug screening.
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