分子生物学
横截
鸟嘌呤
基因
生物
胸腺嘧啶
核酸序列
DNA
胞嘧啶
遗传学
核苷酸
突变
作者
Guanghui Deng,Yi Lü,S M Chen,Jing Miao,Ge Lu,H Li,Hongbo Cai,Xia Xu,Z E,P N Liu
出处
期刊:PubMed
日期:1987-06-15
卷期号:47 (12): 3195-8
被引量:32
摘要
The rat fibroblast cell line Rat 1 was transfected with total DNA of a gastrocarcinoma cell line, BGC-823. The transforming gene was cloned from the genomic library of the secondary transformants using in situ hybridization with a probe of the human Alu repeat sequence. This cloned gene is homologous to the protooncogene c-Ha-ras. The activation lesion of the transforming gene was identified by sequence analysis as a single nucleotide substitution of thymine for guanine in the 12th codon. This results in the substitution of valine for glycine at the 12th amino acid of the Mr 21,000 protein.
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