Effect of Extraction Methods on Polysaccharide of <em>Clitocybe maxima</em> Stipe

Clitocybe maxima (Gartn. ex Mey. Fr.) Quél. is a favorable edible fungi species. The proportion of its stipe is about 45% of entire fruit biomass, which is a low value defined byproduct. To increase its value added utilization, three extraction methods (as hot water, microwave-assisted and complex-enzyme-hydrolysis-assist) were conducted. The extraction effect on the polysaccharide of Clitocybe maxima stipe was compared and the processing conditions in extraction were optimized. The content of polysaccharide was determined with Phenol Sulfuric Acid Method. With three methods, the extraction yield and the polysaccharide amount were different greatly, which were 5.86 and 40.23% for hot water extraction, 9.4 and 52.62% for microware-assist extraction, 10.26 and 53.13% for complex-enzyme-hydrolysis extraction, respectively. Results indicated that the complex-enzyme-hydrolysis-assist extraction was the optimal approach used for extracting polysaccharide from Clitocybe maxima stipe. It showed with the advantages in high efficiency, energy and running time saving.