细胞内
细胞外
生物合成
化学
细胞毒性
透射电子显微镜
氧化应激
生物物理学
硫化镉
纳米颗粒
镉
核化学
生物化学
体外
基因
生物
无机化学
纳米技术
材料科学
有机化学
作者
Daizong Cui,Jianqi Wang,Wei He,Yue Yang,Min Zhao
标识
DOI:10.1016/j.jhazmat.2020.124485
摘要
In the present study, cadmium-based nanoparticles (NPs) were biosynthesized by incubating their precursor salts with E. coli CD-2. Transmission electron microscopy (TEM) revealed the morphology of the NPs and confirmed that the NPs were formed via an intracellular growth. Energy dispersive spectroscopy (EDS) and X-ray diffraction (XRD) determined the elemental composition of the NPs and identified the NPs as CdS. The contents of extracellular Cd2+, intracellular Cd2+ and intracellular CdS NPs were determined during the whole CdS biosynthetic process. The results demonstrated that the contents of Cd2+ and CdS NPs changed during the biosynthetic process. The colony-forming capability test showed that strain CD-2 could maintain its growth during CdS biosynthesis. Protein oxidation levels confirmed that the E. coli cells faced oxidative stress induced both by Cd2+ and CdS. Both Cd2+ and CdS NPs affected the cellular antioxidative system by upregulating related gene expression. However, different pathways might be involved to eliminate ROS induced by Cd2+ ions or CdS NPs, respectively. The expression levels of ef-tu, ftsZ, mutS and dnaK were enhanced together with CdS accumulation, indicating that the cells had to overexpress certain related genes to respond to the NPs-induced stress.
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