化学
分析物
质谱法
质谱成像
基质辅助激光解吸/电离
基质(化学分析)
生物分子
吸附
色谱法
定量分析(化学)
分析化学(期刊)
解吸
基质(水族馆)
有机化学
地质学
海洋学
生物化学
作者
Sanghwan Park,Sook Young Yoon,Hyegi Min,Seung Min Moon,Yoon Ji Choi,Il Shin Kim,Ga Hyang Lee,Min Sun Kim,Jungju Seo,Woonggyu Jung,Chang Young Lee
标识
DOI:10.1021/acs.analchem.9b05316
摘要
Mass spectrometry imaging (MSI) based on matrix-assisted laser desorption/ionization (MALDI) provides information on the identification and spatial distribution of biomolecules. Quantitative analysis, however, has been challenging largely due to heterogeneity in both the size of the matrix crystals and the extraction area. In this work, we present a compartmentalized elastomeric stamp for quantitative MALDI-MSI of adsorbed peptides. Filling the compartments with matrix solution and stamping onto a planar substrate extract and concentrate analytes adsorbed in each compartment into a single analyte-matrix cocrystal over the entire stamped area. Walls between compartments help preserve spatial information on the adsorbates. The mass intensity of the cocrystals directly correlates with the surface coverage of analytes, which enables not only quantitative analysis but estimation of an equilibrium constant for the adsorption. We demonstrate via MALDI-MSI relative quantitation of peptides adsorbed along a microchannel with varying surface coverages.
科研通智能强力驱动
Strongly Powered by AbleSci AI