Comparison of Different Colorectal Cancer With Liver Metastases Models Using Six Colorectal Cancer Cell Lines

结直肠癌 医学 移植 癌症 转移 肿瘤科 病理 淋巴结 内科学 癌症研究
作者
Yuting Xu,Lin Zhang,Qingling Wang,Maojin Zheng
出处
期刊:Pathology & Oncology Research [Springer Science+Business Media]
卷期号:26 (4): 2177-2183 被引量:18
标识
DOI:10.1007/s12253-020-00805-3
摘要

At present, modeling methods of colorectal cancer with liver metastases have significant limitations. Here, we established orthotopic and ectopic hepatic metastases models using six colorectal cancer cell lines to choose an ideal animal model for studying colorectal cancer growth and liver metastases. Luciferin-expressing six colorectal cancer cell lines were used to induce animal models of colorectal cancer with liver metastases by intra-splenic injection or implantation of tumor tissue in the caecum. Tumors growth and metastatic events were observed by bioluminescence imaging. In orthotopic transplantation group, six cell lines all had taken rates of 100% for orthotopic tumors but showed variations in rates of growth. HCT-116 cell developed the 50% liver metastases. However, the ectopic transplantation group achieved higher liver metastatic rate, with the highest frequencies for HCT116 cell (90%) and SW620 cell (77.8%). Furthermore, the time to develop liver metastases and survival rates of bearing-tumor mice were shorter than orthotopic transplantation group. Additionally, six colorectal cancer cell lines resulted in more lymph node metastases in orthotopic transplantation group, whereas produced widespread peritoneal seeding in ectopic transplantation group. Bioluminescence imaging and pathological findings confirmed the growth and metastatic characteristics of tumors. Two animal models of colorectal cancer using six cell lines showed highly variations in rates of growth, survival rates of bearing-tumor mice and frequencies of metastases. The study provides useful information for the establishment of clinically relevant colorectal cancer with liver metastases animal models.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
桐桐完成签到,获得积分0
刚刚
慕青应助finkle采纳,获得10
刚刚
SUN完成签到,获得积分10
1秒前
1秒前
1秒前
1秒前
1秒前
2秒前
3秒前
量子星尘发布了新的文献求助10
3秒前
李李完成签到 ,获得积分10
4秒前
蒲公英发布了新的文献求助20
5秒前
5秒前
LUCCAS发布了新的文献求助20
6秒前
6秒前
三叔完成签到,获得积分0
6秒前
6秒前
Alicia发布了新的文献求助10
7秒前
ossantu发布了新的文献求助10
7秒前
finkle完成签到,获得积分10
8秒前
ZJH发布了新的文献求助10
8秒前
youxiu1112发布了新的文献求助10
8秒前
wwxd完成签到,获得积分10
9秒前
Ryan完成签到,获得积分20
9秒前
Jayce完成签到,获得积分10
10秒前
11秒前
11秒前
11秒前
11秒前
Kuseite完成签到,获得积分10
11秒前
迅速冰岚发布了新的文献求助10
11秒前
LPeaQ应助期待采纳,获得10
11秒前
nan完成签到,获得积分20
12秒前
魔幻代梅发布了新的文献求助10
13秒前
元气饱满完成签到,获得积分10
13秒前
14秒前
FunGuy发布了新的文献求助10
15秒前
简单谷梦发布了新的文献求助10
15秒前
orange完成签到,获得积分10
16秒前
晴天发布了新的文献求助10
16秒前
高分求助中
The Mother of All Tableaux Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 2400
Ophthalmic Equipment Market by Devices(surgical: vitreorentinal,IOLs,OVDs,contact lens,RGP lens,backflush,diagnostic&monitoring:OCT,actorefractor,keratometer,tonometer,ophthalmoscpe,OVD), End User,Buying Criteria-Global Forecast to2029 2000
Optimal Transport: A Comprehensive Introduction to Modeling, Analysis, Simulation, Applications 800
Official Methods of Analysis of AOAC INTERNATIONAL 600
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 588
T/CIET 1202-2025 可吸收再生氧化纤维素止血材料 500
Interpretation of Mass Spectra, Fourth Edition 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3951758
求助须知:如何正确求助?哪些是违规求助? 3497124
关于积分的说明 11086059
捐赠科研通 3227597
什么是DOI,文献DOI怎么找? 1784497
邀请新用户注册赠送积分活动 868586
科研通“疑难数据库(出版商)”最低求助积分说明 801154