丝素
自愈水凝胶
组织工程
生物相容性
3D生物打印
光引发剂
生物医学工程
光致聚合物
丝绸
材料科学
生物相容性材料
纳米技术
化学
聚合
聚合物
复合材料
高分子化学
单体
冶金
医学
作者
Soon Hee Kim,Heesun Hong,Olatunji Ajiteru,Md. Tipu Sultan,Young‐Jin Lee,Ji Seung Lee,Ok Joo Lee,Hanna Lee,Hae Sang Park,Kyu Young Choi,Joong Seob Lee,Hyung Woo Ju,In–Sun Hong,Chan Hum Park
出处
期刊:Nature Protocols
[Springer Nature]
日期:2021-10-29
卷期号:16 (12): 5484-5532
被引量:149
标识
DOI:10.1038/s41596-021-00622-1
摘要
The development of biocompatible and precisely printable bioink addresses the growing demand for three-dimensional (3D) bioprinting applications in the field of tissue engineering. We developed a methacrylated photocurable silk fibroin (SF) bioink for digital light processing 3D bioprinting to generate structures with high mechanical stability and biocompatibility for tissue engineering applications. Procedure 1 describes the synthesis of photocurable methacrylated SF bioink, which takes 2 weeks to complete. Digital light processing is used to fabricate 3D hydrogels using the bioink (1.5 h), which are characterized in terms of methacrylation, printability, mechanical and rheological properties, and biocompatibility. The physicochemical properties of the bioink can be modulated by varying photopolymerization conditions such as the degree of methacrylation, light intensity, and concentration of the photoinitiator and bioink. The versatile bioink can be used broadly in a range of applications, including nerve tissue engineering through co-polymerization of the bioink with graphene oxide, and for wound healing as a sealant. Procedure 2 outlines how to apply 3D-printed SF hydrogels embedded with chondrocytes and turbinate-derived mesenchymal stem cells in one specific in vivo application, trachea tissue engineering, which takes 2–9 weeks.
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