Chitosan-based particles for protection of proteins during storage and oral administration

Abstract Crosslinked chitosan particles have been developed to improve the storage stability of bioactive proteins and to ensure adequate bioavailability by targeting release to the colon. The characteristics of these chitosan-based particles, formed using different molecular weight chitosans and different anion cross-linkers, were demonstrated using small angle X-ray scattering, ζ-potential, particle size, and morphology. An in vitro release study showed that protein release from the particles in the upper gastrointestinal tract was