电穿孔
材料科学
摩擦电效应
纳米技术
纳米发生器
药物输送
透皮
纳米针
细胞内
生物物理学
细胞生物学
压电
化学
生物
复合材料
纳米结构
基因
药理学
生物化学
作者
Zhirong Liu,Jinhui Nie,Bin Miao,Jiadong Li,Yuanbo Cui,Shu Wang,Xiaodi Zhang,Gengrui Zhao,Yongbo Deng,Yihui Wu,Zhou Li,Linlin Li,Zhong Lin Wang
标识
DOI:10.1002/adma.201807795
摘要
Nondestructive, high-efficiency, and on-demand intracellular drug/biomacromolecule delivery for therapeutic purposes remains a great challenge. Herein, a biomechanical-energy-powered triboelectric nanogenerator (TENG)-driven electroporation system is developed for intracellular drug delivery with high efficiency and minimal cell damage in vitro and in vivo. In the integrated system, a self-powered TENG as a stable voltage pulse source triggers the increase of plasma membrane potential and membrane permeability. Cooperatively, the silicon nanoneedle-array electrode minimizes cellular damage during electroporation via enhancing the localized electrical field at the nanoneedle-cell interface and also decreases plasma membrane fluidity for the enhancement of molecular influx. The integrated system achieves efficient delivery of exogenous materials (small molecules, macromolecules, and siRNA) into different types of cells, including hard-to-transfect primary cells, with delivery efficiency up to 90% and cell viability over 94%. Through simple finger friction or hand slapping of the wearable TENGs, it successfully realizes a transdermal biomolecule delivery with an over threefold depth enhancement in mice. This integrated and self-powered system for active electroporation drug delivery shows great prospect for self-tuning drug delivery and wearable medicine.
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