膜
血凝素(流感)
螺旋线圈
肽
生物物理学
三聚体
脂质双层融合
化学
小泡
甲型流感病毒
病毒膜
病毒包膜
生物化学
生物
病毒
病毒学
糖蛋白
二聚体
有机化学
作者
Ye Yu,David S. King,Yeon‐Kyun Shin
出处
期刊:Science
[American Association for the Advancement of Science (AAAS)]
日期:1994-10-14
卷期号:266 (5183): 274-276
被引量:104
标识
DOI:10.1126/science.7939662
摘要
The trimeric protein hemagglutinin (HA) of the influenza viral envelope is essential for cell entry. To investigate the interaction of HA with membranes, two 40-residue, cysteine-substituted peptides comprising the loop region and the first part of the coiled-coil stem were synthesized and modified with a nitroxide spin label. Electron paramagnetic resonance analysis revealed that the peptide inserts reversibly into phospholipid vesicles under endosomal pH conditions. This result suggests that some or all of the long coiled-coil trimer of HA may insert into membranes, which could bring the viral and cell membranes closer together and facilitate fusion.
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