脱磷
酶
磷酸酶
碱性磷酸酶
磷酸单酯酶
生物化学
化学
作者
Michael R. Green,Joseph Sambrook
出处
期刊：CSH Protocols
[Cold Spring Harbor Laboratory Press]
日期：2020-08-01
卷期号：2020 (8): pdb.top100768-pdb.top100768
被引量：29
标识
DOI：10.1101/pdb.top100768
摘要
Several types of alkaline phosphatases (or alkaline phosphomonoesterase) are commonly used in molecular cloning, including bacterial alkaline phosphatase (BAP) and calf intestinal alkaline phosphatase (CIP, CIAP, or CAP). Similar enzymes isolated from more esoteric cold-blooded organisms (e.g., SAP from shrimp) have become available in recent years and have the advantage of being easier to inactivate than BAP or CIP at the end of dephosphorylation reactions. The uses and properties of these enzymes are introduced here.
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