Circulating Microvesicles in Convalescent Ischemic Stroke Patients: A Contributor to High-On-Treatment Residual Platelet Reactivity?

氯吡格雷 医学 血小板 微泡 冲程(发动机) 全血 内科学 缺血性中风 心脏病学 血沉 阿司匹林 缺血 化学 机械工程 小RNA 生物化学 工程类 基因
作者
Diana Schrick,Tihamér Molnár,Margit Tökés‐Füzesi,Abigél Molnár,Erzsébet Ezer
出处
期刊:Frontiers in bioscience [Bioscience Research Institute Pte. Ltd.]
卷期号:27 (5) 被引量:3
标识
DOI:10.31083/j.fbl2705158
摘要

Introduction: Exploration of novel and effective antiplatelet strategies for the secondary prevention of ischemic stroke is utmost. Some platelet derived microparticles (PMVs) in convalescent stroke subjects were found to be predictive for the next vascular event. Patients with high-on-treatment platelet reactivity (HTPR) had a significantly higher risk for ischemic stroke. Here, we aimed to explore associations among circulating microparticles and responsivness to antiplatelet (clopidogrel) therapy. Methods: A total of 18 patients on clopidogrel therapy due to secondary stroke prevention were rospectively recruited into this study. Twenty age-matched healthy subjects served as controls. Flow cytometric measurements of microparicles (MVs) and data analysis were performed on Beckman-Coulter FC-500 cytometer with CXP software. Besides, platelet aggregometry data were revealed. Both measurements were performed in whole blood and from the lower and upper blood fractions separated after 1-hour gravity sedimentation by the analogy with erythrocyte sedimentation rate. Results: The total number of circulating MVs, and particularly the platelet derived CD42+ and PAC-1+ were significantly higher in post-stroke patients (p < 0.001). The platelet aggregation in the whole blood (area under the curve, AUC) showed a significant negative correlation with the total number of MPs in the lower blood sample after 1-hour gravity sedimentation (r = –0.650, p = 0.005). Next, we analyzed associations among MPs and aggregometry data obtained from clopidogrel responders and non-responders. Both, area under the curve (AUC) and velocity in the whole blood showed opposite correlation with the total number of MVs in the lower blood sample after 1-hour gravity sedimentation. Importantly, a significant negative correlation was observed for the velocity (r = –0.801, p = 0.005), but not for the AUC in responders. Platelet derived CD42+ and PAC-1+ MVs showed positive correlations with neutrophils in the lower blood sample (p = 0.008 and p = 0.006 respectively). Conclusions: Circulating MVs may allow to monitor the response to antiplatelet therapy in post-stroke patients. In addition, the link between platelet derived MVs and neutrophil granulocytes might become therapeutic targets in the future.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
1秒前
1秒前
2秒前
3秒前
研友_V8Qmr8发布了新的文献求助10
3秒前
3秒前
Bilipear发布了新的文献求助10
4秒前
4秒前
wanci应助隔壁庞仨儿采纳,获得10
4秒前
5秒前
5秒前
勤恳山晴完成签到,获得积分10
6秒前
gww完成签到,获得积分10
7秒前
菜鸟发布了新的文献求助10
7秒前
7秒前
keleboys发布了新的文献求助10
8秒前
SLY完成签到 ,获得积分10
9秒前
林木木发布了新的文献求助30
9秒前
gww发布了新的文献求助10
10秒前
奋斗凌珍发布了新的文献求助10
10秒前
11秒前
12秒前
Bilipear完成签到,获得积分10
13秒前
wang发布了新的文献求助10
16秒前
JamesPei应助Kkk采纳,获得10
16秒前
17秒前
18秒前
123发布了新的文献求助30
18秒前
20秒前
赘婿应助Asnirelia采纳,获得30
21秒前
打老虎发布了新的文献求助10
22秒前
22秒前
22秒前
22秒前
23秒前
ccc7完成签到 ,获得积分10
25秒前
25秒前
25秒前
深情安青应助zz采纳,获得10
26秒前
高分求助中
Continuum thermodynamics and material modelling 3000
Production Logging: Theoretical and Interpretive Elements 2500
Healthcare Finance: Modern Financial Analysis for Accelerating Biomedical Innovation 2000
Applications of Emerging Nanomaterials and Nanotechnology 1111
Les Mantodea de Guyane Insecta, Polyneoptera 1000
Theory of Block Polymer Self-Assembly 750
지식생태학: 생태학, 죽은 지식을 깨우다 700
热门求助领域 (近24小时)
化学 医学 材料科学 生物 工程类 有机化学 生物化学 纳米技术 内科学 物理 化学工程 计算机科学 复合材料 基因 遗传学 物理化学 催化作用 细胞生物学 免疫学 电极
热门帖子
关注 科研通微信公众号,转发送积分 3483822
求助须知:如何正确求助?哪些是违规求助? 3073054
关于积分的说明 9129181
捐赠科研通 2764683
什么是DOI,文献DOI怎么找? 1517299
邀请新用户注册赠送积分活动 702065
科研通“疑难数据库(出版商)”最低求助积分说明 700880