志贺氏菌
检出限
化学
磁性纳米粒子
生物传感器
荧光
适体
辣根过氧化物酶
色谱法
纳米颗粒
纳米技术
材料科学
分子生物学
生物化学
生物
大肠杆菌
酶
物理
量子力学
基因
作者
Yaqi Song,Min Chen,Zhongyu Yan,Lu Han,Leiqing Pan,Kang Tu
出处
期刊:Chemosensors
[MDPI AG]
日期:2023-05-20
卷期号:11 (5): 309-309
被引量:1
标识
DOI:10.3390/chemosensors11050309
摘要
Shigella, a typical and fatal foodborne pathogen with strong infectivity and survivability in foodstuff, demands a simple and sensitive detecting method. In this study, we reported a novel nanoplatform based on biofunctionalized magnetic nanoparticles (MNPs) modified upconversion nanoparticles (UCNPs) for rapid and specific determination of Shigella. Due to base pairing, Shigella aptamer-functionalized horseradish peroxidase (HRP) combined with complementary strand-modified MNPs@UCNPs. In the absence of Shigella, HRP associated with MNPs@UCNPs were magnetically separated, and colorless 3,3′,5,5′-tetramethylbenzidine (TMB) was oxidized into blue oxTMB. The overlap between oxTMB’s absorption peak and MNPs@UCNPs’ emission peak caused the fluorescence quenching at 545 nm. The MNPs@UCNPs fluorescence biosensor was achieved to detect Shigella in 1 h, with a limit of detection of 32 CFU/mL. This work showed a rapid and specific sensing platform and produced satisfactory chicken sample results.
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