Pharmacodynamic and metabonomic studies on the improvement of DR with Fufang Xueshuantong component compatibility

The Fufang Xueshuantong capsule (FXT) has significant preventive and therapeutic effects on diabetic retinopathy, however, its active ingredient compatibility still needs to be explored in depth. In this study, a zebrafish diabetic retinopathy model was established using high-mixed sugars, and the optimal ratios of notoginseng total saponins, total salvianolic acid, astragaloside, and harpagide were selected through orthogonal experiments. Using UPLC-QqQ/MS to detect the changes in amino acid content in DR zebrafish tissues after administration of FXT and compatible formula, to analyze the effects of FXT and compatible formula on amino acid metabolites. The results show that the final compatibility ratios of the components were 8: 5: 1: 6.6 by comprehensive evaluation of the indicators. FXT and its compatibility formula had beneficial effects on retinal vasodilatation, lipid accumulation in the liver, total glucose, and VEGF levels in DR zebrafish, and all of them could call back some amino acid levels in DR zebrafish. In the present study, we determined the compatibility formulation of the Fufang Xueshuantong capsule and investigated its efficacy in DR zebrafish to provide a reference for further clinical application.