严重急性呼吸综合征冠状病毒2型(SARS-CoV-2)
荧光
金标准(测试)
灵敏度(控制系统)
2019年冠状病毒病(COVID-19)
实时聚合酶链反应
病毒学
病毒
材料科学
化学
生物
医学
物理
光学
基因
病理
放射科
生物化学
传染病(医学专业)
疾病
电子工程
工程类
作者
Chunsen Zhu,Hao Fang,Houshi Ma,Jianya Xue,Zeqin Li,Xi Wu,Gangyin Luo
摘要
ABSTRACT Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has infected many people around the world; fast and accurate detection of the virus can help control the spread of the virus. Reverse transcription-polymerase chain reaction (RT-PCR) is the gold standard method for SARS-CoV-2 detection. In this study, we improved the RT-PCR by proposing a novel method using dual double-quenched fluorescence probes. We used the improved probes to detect the plasmid DNA and RNA reference materials of SARS-CoV-2, respectively. The results show that, the background fluorescence intensity reduced by 50%, the fluorescence increment increased to 2.8 folds, and the Ct value significantly reduced by 3 or more, indicating that the detection sensitivity increased at least 8 times. In addition, we demonstrated that the improved probes have well performance in detecting SARS-CoV-2, with the minimum concentration of 6.2 copies/µL. This study will help biological companies develop better products for SARS-CoV-2 and other clinical pathogen infection.
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