黄病毒
病毒学
生物
黄病毒科
病毒
白纹伊蚊
免疫
西尼罗河病毒
脑炎
免疫学
抗体
病毒性疾病
埃及伊蚊
幼虫
植物
作者
Hongqing Zhang,Na Li,Zherui Zhang,Cheng‐Lin Deng,Han Xia,Han‐Qing Ye,Zhiming Yuan,Bo Zhang
标识
DOI:10.1093/infdis/jiad238
摘要
Abstract West Nile virus (WNV), an arthropod-borne flavivirus, can cause severe symptoms, including encephalitis, and death, posing a threat to public health and the economy. However, there is still no approved treatment or vaccine available for humans. Here, we developed a novel vaccine platform based on a classical insect-specific flavivirus (cISF) YN15-283-02, which was derived from Culicoides. The cISF-WNV chimera was constructed by replacing prME structural genes of the infectious YN15-283-02 cDNA clone with those of WNV and successfully rescued in Aedes albopictus cells. cISF-WNV was nonreplicable in vertebrate cells and nonpathogenic in type I interferon receptor (IFNAR)-deficient mice. A single-dose immunization of cISF-WNV elicited considerable Th1-biased antibody responses in C57BL/6 mice, which was sufficient to offer complete protection against lethal WNV challenge with no symptoms. Our studies demonstrated the potential of the insect-specific cISF-WNV as a prophylactic vaccine candidate to prevent infection with WNV.
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