精子发生
果蝇属(亚属)
细胞生物学
物理
计算机科学
生物
遗传学
基因
内分泌学
作者
Elham Alzyoud,Dániel Németh,Viktor Vedelek,Titanilla Szögi,Viktória Tóth,Zoltán Lipinszki,Rita Sinka,Zoltán Lipinszki,Rita Sinka
标识
DOI:10.1101/2024.03.20.585862
摘要
Abstract The initiation of microtubule formation is facilitated by γ-tubulin and γ-Tubulin Ring Complex (γ-TuRC) in various microtubule-organizing centers (MTOCs). While the heterogeneity of tissue-specific MTOCs and γ-TuRC in Drosophila testis has been described, their molecular composition and physiological significance are poorly understood. We investigated the testis-specific distribution and biochemical interaction of the canonical γ-TuRC proteins Grip163 and Grip84. We found that while Grip163 is present on the centrosome and basal body, Grip84 localizes to the centrosome and Golgi in spermatocytes and colocalizes with the testis-specific γ-TuRC at the basal body, apical nuclear tip, and near the elongated mitochondria after meiosis. We also show the apical nuclear tip localization of some γ-TuRC interacting partners and prove their binding to testis-specific γ-TuRC proteins. These results highlight and prove the importance of the different γ-TuRCs in organizing the diverse MTOCs present during the extensive rearrangement of cell organelles during the spermatogenesis of Drosophila .
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