粘蛋白
乳腺癌
生物标志物
聚糖
癌症研究
癌症
流式细胞术
糖基化
糖蛋白
微泡
化学
生物
分子生物学
生物化学
医学
内科学
小RNA
基因
作者
Saroj Saroj,Debashish Paul,Akbar Ali,Chrysafis Andreou,Soumik Pal,Tatini Rakshit
出处
期刊:ACS applied bio materials
[American Chemical Society]
日期:2023-10-12
卷期号:6 (11): 4944-4951
被引量:3
标识
DOI:10.1021/acsabm.3c00651
摘要
Aberrantly glycosylated mucin 1 is a critical prognostic biomarker in breast epithelial cancers. Hypoglycosylated mucin 1 coats the surface of the cancer cells, where O-glycans are predominantly linked via an N-acetylgalactosamine moiety (GalNAc). Cancer cell-derived extracellular vesicles (EVs) carry biomarkers from parent cancer cells to the recipient cells and, therefore, could potentially be leveraged for diagnostics and noninvasive disease monitoring. We devised a label-free approach for identifying glycoprotein mucin 1 overexpression on breast cancer EVs. While exploring a plethora of biochemical (enzyme-linked immunosorbent assay, flow cytometry, and SDS-PAGE) and label-free biophysical techniques (circular dichroism and infrared spectroscopy (IR)) along with multivariate analysis, we discovered that mucin 1 is significantly overexpressed in breast cancer EVs and aberrant glycosylation in mucin 1 could be critically addressed using IR and multivariate analysis targeting the GalNAc sugar. This approach emerges as a convenient and comprehensive method of distinguishing cancer EVs from normal samples and holds potential for nonintrusive breast cancer liquid biopsy screening.
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