再生(生物学)
静电纺丝
聚己内酯
雪旺细胞
纳米纤维
材料科学
神经组织工程
生物相容性
组织工程
神经导管
生物医学工程
基质(化学分析)
化学
纳米技术
复合材料
解剖
细胞生物学
聚合物
冶金
生物
医学
作者
Yas Al-Hadeethi,Aishwarya Nagarajan,Srividya Hanuman,Hiba Mohammed,Aakanksha M. Vetekar,Goutam Thakur,Le Dinh,Yin Yao,E.M. Mkawi,Mahmoud A. Hussein,Vipul Agarwal,Manasa Nune
出处
期刊:RSC Advances
[The Royal Society of Chemistry]
日期:2023-01-01
卷期号:13 (2): 1392-1401
被引量:1
摘要
Nerve tissue engineering aims to create scaffolds that promote nerve regeneration in the damaged peripheral nervous system. However, there remain some challenges in the construction of scaffolds in terms of mechanical properties and cellular behaviour. The present work aims to develop multifunctional implantable nanofibrous scaffolds for nerve regeneration. Using electrospinning, nanofibrous neat polycaprolactone (PCL) and PCL/multiwalled carbon nanotubes (PCL-MWCNT) composite scaffolds were prepared in random and aligned morphology. Schwann cells and their secreted biochemical factors are responsible for neuronal survival in the peripheral nervous system. Therefore, the acellular matrix of Schwann cells was spin-coated on the PCL-MWCNT scaffolds to aid nerve regeneration. Physicochemical and mechanical properties, and the in vitro cellular response of the developed nanofibrous were investigated. We observed no significant change in fibre diameter between neat PCL and PCL-MWCNT scaffolds regardless of the morphology. However, the inclusion of MWCNT reduced the mechanical strength of nanocomposite scaffolds compared to neat PCL. In vitro study revealed biocompatibility of the developed scaffolds both with and without an acellular matrix. Gene expression study revealed a significant increase in peripheral myelin protein (PMP22) expression on acellular matrix-coated PCL-MWCNT scaffolds compared to neat PCL counterparts. Overall, the results suggested Schwann cell matrix-coated PCL-MWCNT nanofibers as a promising conduit for peripheral nerve regeneration.
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