Transcriptomic Analysis of Differentially Expressed Genes in Arabidopsis thaliana Overexpressing BnMYB2 from Boehmeria nivea under Cadmium Stress

Boehmeria nivea (ramie) is an important fiber crop with strong tolerance to cadmium (Cd). In our previous study, a novel MYB transcription factor gene from ramie, BnMYB2, was found to positively regulate Cd tolerance and accumulation in the transgenic Arabidopsis thaliana lines. Herein, transcriptome sequencing was performed to identify the differentially expressed genes involved in cadmium response between the wild-type (WT) and BnMYB2 overexpressed lines; 1598 differentially expressed genes (DEGs) were detected in the shoot. GO and KEGG analysis indicated that the majority of DEGs belonged to the categories of transcription factors, plant hormone signal transduction and nitrogen metabolism. The expression level of the Ib subgroup bHLH genes (AtbHLH38, AtbHLH39, AtbHLH100 and AtbHLH101) and nitrogen assimilation-related genes (AtNIA1, AtNIA2, AtNIR1 and AtASN2) were significantly higher than that of WT, accompanied with the positive changes in iron (Fe) and total nitrogen content in the shoot of BnMYB2 overexpression lines. Several DEGs belonging to the bZIP transcription factor family or SAUR family were also found up-regulated in the transgenic plants. These results provide important clues for elucidating how the molecular mechanisms of BnMYB2 regulate plant response to Cd stress.