A Manganese‐Based Metal–Organic Framework as a Cold‐Adapted Nanozyme

Abstract The development of cold‐adapted enzymes with high efficiency and good stability is an advanced strategy to overcome the limitations of catalytic medicine in low and cryogenic temperatures. In this work, inspired by natural enzymes, a novel cold‐adapted nanozyme based on a manganese‐based nanosized metal–organic framework (nMnBTC) is designed and synthesized. The nMnBTC as an oxidase mimetic not only exhibits excellent activity at 0 °C, but also presents almost no observable activity loss as the temperature is increased to 45 °C. This breaks the traditional recognition that enzymes show maximum activity only under specific psychrophilic or thermophilic condition. The superior performance of nMnBTC as a cold‐adapted nanozyme can be attributed to its high‐catalytic efficiency at low temperature, good substrate affinity, and flexible conformation. Based on the robust performance of nMnBTC, a low‐temperature antiviral strategy is developed to inactivate influenza virus H1N1 even at −20 °C. These results not only provide an important guide for the rational design of highly efficient artificial cold‐adapted enzymes, but also pave a novel way for biomedical application in cryogenic fields.