Thalidomide suppresses migration and invasion of colorectal cancer cells by inhibiting HOXB7‐mediated activation of the Wnt/β‐catenin signaling pathway

活力测定 Wnt信号通路 细胞凋亡 细胞迁移 癌症研究 流式细胞术 下调和上调 生物 转染 连环素 信号转导 细胞 细胞培养 免疫学 细胞生物学 基因 生物化学 遗传学
作者
Liyang Liu,Wusong Xue
出处
期刊:Chemical Biology & Drug Design [Wiley]
卷期号:103 (1)
标识
DOI:10.1111/cbdd.14434
摘要

Abstract Heaps of studies have verified the effects of thalidomide (THA) on colorectal cancer (CRC). Howbeit, the corresponding mechanism awaits illustration, which is the foothold of this study. Following the treatment of 0, 1.94, 7.75, or 19.36 μM THA, CRC cell viability, apoptosis, migration, and invasion were evaluated by methyl tetrazolium, flow cytometry, wound‐healing, and transwell assays. Homeobox B7 (HOXB7) expression in CRC was analyzed and detected by bioinformatics analysis, quantitative real‐time PCR or western blot. After the corresponding transfection or treatment with inhibitor of catenin‐responsive transcription‐3 (iCRT‐3), abovementioned CRC cell biological behaviors as well as expression levels of HOXB7 and β‐catenin were evaluated. 7.75 and 19.36 μM THA dwindled CRC cell viability, migration, and invasion, and facilitated apoptosis. HOXB7 upregulation was detected in CRC cells, which promoted the viability, migration, invasion, and β‐catenin expression, and weakened the apoptosis of CRC cells. Also, HOXB7 upregulation counteracted the effects of THA on CRC cells. iCRT‐3 restrained β‐catenin expression, viability, migration, and invasion, whereas promoting the apoptosis of CRC cells. In addition, iCRT‐3 antagonized the effects of overexpressed HOXB7 on CRC cells. THA inhibits the migration and invasion of CRC cells, which is achieved by suppressing HOXB7‐mediated activation of Wnt/β‐catenin signaling pathway.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
千跃应助科研通管家采纳,获得10
刚刚
笨笨中心应助科研通管家采纳,获得10
刚刚
CodeCraft应助科研通管家采纳,获得10
刚刚
今后应助科研通管家采纳,获得10
刚刚
刚刚
刚刚
千跃应助科研通管家采纳,获得10
1秒前
1秒前
大模型应助kmo采纳,获得10
3秒前
悦悦发布了新的文献求助10
3秒前
静槐发布了新的文献求助10
4秒前
Han发布了新的文献求助10
4秒前
5秒前
5秒前
Ava应助竹林听风采纳,获得10
5秒前
orixero应助八九采纳,获得10
5秒前
阙飘飘发布了新的文献求助10
6秒前
6秒前
搞怪文轩完成签到,获得积分10
7秒前
7秒前
8秒前
alexa发布了新的文献求助10
9秒前
dpp发布了新的文献求助10
9秒前
科目三应助可爱青曼采纳,获得30
9秒前
量子星尘发布了新的文献求助10
9秒前
邹长飞发布了新的文献求助10
11秒前
12秒前
文献直达发布了新的文献求助10
12秒前
CUREME完成签到,获得积分10
13秒前
小透明发布了新的文献求助10
13秒前
Homura完成签到,获得积分10
14秒前
16秒前
Yajuan关注了科研通微信公众号
17秒前
17秒前
zwd完成签到 ,获得积分10
17秒前
dpp完成签到,获得积分10
18秒前
幽默的妍完成签到 ,获得积分10
18秒前
18秒前
18秒前
快让我滚蛋毕业完成签到,获得积分10
19秒前
高分求助中
The Mother of All Tableaux Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 2400
Ophthalmic Equipment Market by Devices(surgical: vitreorentinal,IOLs,OVDs,contact lens,RGP lens,backflush,diagnostic&monitoring:OCT,actorefractor,keratometer,tonometer,ophthalmoscpe,OVD), End User,Buying Criteria-Global Forecast to2029 2000
A new approach to the extrapolation of accelerated life test data 1000
Cognitive Neuroscience: The Biology of the Mind 1000
Cognitive Neuroscience: The Biology of the Mind (Sixth Edition) 1000
Optimal Transport: A Comprehensive Introduction to Modeling, Analysis, Simulation, Applications 800
Official Methods of Analysis of AOAC INTERNATIONAL 600
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3959477
求助须知:如何正确求助?哪些是违规求助? 3505697
关于积分的说明 11125320
捐赠科研通 3237538
什么是DOI,文献DOI怎么找? 1789202
邀请新用户注册赠送积分活动 871583
科研通“疑难数据库(出版商)”最低求助积分说明 802868